{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Status of the Thalamus Corpus\n",
    "\n",
    "This notebook compute and display some useful information about the status of the thalamus corpus."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [],
   "source": [
    "## Installation of the Neuro Annotation Toolbox (NAT)\n",
    "#!pip3 install -q --upgrade  git+https://github.com/BlueBrain/nat.git --user #--force-reinstall\n",
    "    \n",
    "## Installation of other standard toolboxes\n",
    "#!pip3 install -q matplotlib seaborn #--user"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [],
   "source": [
    "## Downloading the Thalamus corpus\n",
    "from git import Repo\n",
    "import os\n",
    "\n",
    "git_url = \"https://github.com/christian-oreilly/corpus-thalamus.git\"\n",
    "repo_dir = os.path.join(os.getcwd(), \"neurocuratorDB\")\n",
    "if not os.path.isdir(repo_dir):\n",
    "    Repo.clone_from(git_url, repo_dir)\n",
    "else:\n",
    "    Repo(repo_dir).remotes.origin.pull()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {},
   "outputs": [],
   "source": [
    "from glob import glob\n",
    "\n",
    "from nat.annotation import Annotation\n",
    "from nat.treeData import flatten_list\n",
    "\n",
    "annotations = []\n",
    "for fileName in glob(repo_dir + \"/*.pcr\"):\n",
    "    try:\n",
    "        annotations.extend(Annotation.readIn(open(fileName, \"r\", encoding=\"utf-8\", errors='ignore')))\n",
    "    except:       \n",
    "        print(\"Skipping: \", fileName)    \n",
    "        raise"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Nb. annotations</th>\n",
       "      <th>Nb. papers</th>\n",
       "      <th>Nb. parameters</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>582</td>\n",
       "      <td>113</td>\n",
       "      <td>485</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "   Nb. annotations  Nb. papers  Nb. parameters\n",
       "0              582         113             485"
      ]
     },
     "execution_count": 4,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "import pandas as pd\n",
    "import numpy as np\n",
    "\n",
    "pd.DataFrame({\"Nb. annotations\" : [len(annotations)],\n",
    "              \"Nb. papers\"      : [len(np.unique([annot.pubId for annot in annotations]))],\n",
    "              \"Nb. parameters\"  : [len(flatten_list([[(param, annot) for param in annot.parameters]  \n",
    "                                                     for annot in annotations]))]})"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {},
   "outputs": [],
   "source": [
    "parameters = flatten_list([annot.parameters for annot in annotations])"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "image/png": 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\n",
      "text/plain": [
       "<matplotlib.figure.Figure at 0x7f6c14295c50>"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "%matplotlib inline\n",
    "import seaborn as sb\n",
    "import pandas as pd\n",
    "import matplotlib.pyplot as plt\n",
    "from nat.modelingParameter import getParameterTypeNameFromID\n",
    "\n",
    "plt.figure(figsize=(10, 2))\n",
    "\n",
    "paramTypes = pd.Series([param.typeId for param in parameters])\n",
    "\n",
    "axes = sb.countplot(paramTypes, orient='h',\n",
    "                    order=paramTypes.value_counts().iloc[:30].index)\n",
    "\n",
    "#plt.xticks(plt.xticks(), plt.gca().get_xticklabels(), rotation='vertical')\n",
    "paramNames = [getParameterTypeNameFromID(typeId.get_text()) for typeId in axes.get_xticklabels()]\n",
    "axes.set_xticklabels(paramNames, rotation='vertical')\n",
    "pass\n",
    "\n",
    "plt.savefig(\"annotatedParamHist.png\", bbox_inches='tight', dpi=300)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Printing information for the most annotated parameters"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "metadata": {
    "scrolled": false
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "PARAMETER NAME: conductance_ion_curr_max\n",
      "Number:  1\n",
      "Text:  e Na ϩ and K ϩ currents responsible for fast action poten- tials, they were inserted in the soma, and their kinetics were taken from a model of hippocampal pyramidal cells (Traub and Miles, 1991), assum- ing a resting potential of V T ϭ Ϫ52 mV in their equations, maximal conductances of g ៮ Na ϭ 100 mS/cm 2 and g ៮ K ϭ 100 mS/cm 2 , and reversal potentials of E Na ϭ 50 mV and E K ϭ Ϫ100 mV\n",
      "Context:  t currents. Voltage-dependent conductances were modeled using a Hodgkin –Huxley type of kinetic model (Hodgkin and Huxley, 1952). Because no data exist to constrain the localization and kinetics of the Na ϩ and K ϩ currents responsible for fast action potentials, they were inserted in the soma, and their kinetics were taken from a model of hippocampal pyramidal cells (Traub and Miles, 1991), assuming a resting potential of VT ϭ Ϫ52 mV in their equations, maximal conductances of g៮ Na ϭ 100 mS/cm 2 and g៮ K ϭ 100 mS/cm 2, and reversal potentials of ENa ϭ 50 mV and EK ϭ Ϫ100 mV. This model was already shown to be adequate to model the repetitive firing within bursts of action potentials (Traub and Miles, 1991; Destexhe et al., 1996a,b). The kinetics of activation and inactivation of \n",
      "Values:  100.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  2\n",
      "Text:  current were chosen to be 500 and 100 pS/lm2, respectively, and the peak conductance density for the non-inactivating K+ current was chosen to be 5 pS/lm2.\n",
      "Context:  amics of synapses according to the type (excitatory or inhibitory) of the pre- and postsynaptic neuron: mean values of U, D, and F according to Markram and others (1998) and Gupta and others (2000).  current were chosen to be 500 and 100 pS/lm2, respectively, and the + peak conductance density for the non-inactivating K current was 2 chosen to be 5 pS/lm . The membrane area of the neuron was set to be 34 636 lm2 as in Destexhe and others (2001). For each simulation, the initial conditions of each neuron, that is, the membrane voltage at time t = 0, \n",
      "Values:  100.0\n",
      "Unit:  pS/um**2\n",
      " \n",
      "Number:  3\n",
      "Text:  lthough we observed a typical T-type Ca2` current in more than 80% of our sample of interneurones (n = 34), it was significantly smaller in size (mean maximal amplitude, 109 +/- 46 pA; n = 11) than that in relay cells (289 +/- 131 pA; n = 16)\n",
      "Context:   & Pape, 1989), decreased in size after equimolar substitution of extracellular Ca2\" by barium ions (Ba2+), and was readily blocked by addition of 50 /SM Ni2` to the bathing medium (data not shown). Although we observed a typical T-type Ca2` current in more than 80% of our sample of interneurones (n = 34), it was significantly smaller in size (mean maximal amplitude, 109 + 46 pA; n = 11) than that in relay cells (289 + 131 pA; n = 16), and the steady-state voltage dependence of IT was different in the two types of LGN neurones. Figure 5 illustrates experiments in which activation and inactivation properties of IT were analysed more qu\n",
      "Values:  289.0 +/- 131.0  (n=16.0)\n",
      "Unit:  pA\n",
      " \n",
      "Number:  4\n",
      "Text:  The gCAN had a conductance of 250\u0002S/cm2\n",
      "Context:  150 ␮s/cm 2, and gH had a reversal potential of ⫺45 mV and peak conductance of 150 ␮s/cm 2. T-type Ca2⫹ channels ( gT) were modeled with a reversal potential of 120 mV and a permeability of 0.7 ␮m/s. The gCAN had a conductance of 250 ␮S/cm 2 and reversal potential of ⫺20 mV. The gKIR and the fast voltage-gated potassium channel ( gKf) had conductances of 20 ␮s/ cm 2 and 50 mS/cm 2, respectively. Both potassium channels had reversal pot\n",
      "Values:  250.0\n",
      "Unit:  uS/cm^2\n",
      " \n",
      "Number:  5\n",
      "Text:  The Na+ and K+ currents responsible for fast action potentials were inserted in the soma, and their kinetics was taken from a model of hippocampal pyra- midal cells (Traub and Miles, 1991), assuming a resting potential of -67 mV in their equations, maximal conductances of & = 100 mS/cm’ and & = 80 mS/cm*, and reversal potentials of E,, = 50 mV and E, = - 100 mV\n",
      "Context:  e, because the model included only a subset of the currents present in these cells. Voltage-dependent conductances were modeled using a HodgkinHuxley type of kinetic model (Hodgkin and Huxley, 1952). The Na+ and K+ currents responsible for fast action potentials were inserted in the soma, and their kinetics was taken from a model of hippocampal pyramidal cells (Traub and Miles, 1991), assuming a resting potential of -67 mV in their equations, maximal conductances of & = 100 mS/cm’ and & = 80 mS/cm*, and reversal potentials of E,, = 50 mV and E, = - 100 mV. The kinetics of activation and inactivation of I, in RE cells, as well as the activation curves of I,, obtained from voltage-clamp recordings in acutely dissociated RE cells (Huguenard and Prince, 199\n",
      "Values:  80.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  6\n",
      "Text:  The amplitudes of Ih currents exhibited a scattered distribution between 12.8 and 159.5 pA, with a mean value of 56.6 +/- 32.1 pA (Fig. 7B). This gave a maximal Ih conductance of 1.02 nS, assuming its reversal potential to be -44 mV {i.e. g = I/(Vm - Eh ) = -57 pA/ [-100 mV - (-44 mV)] = 1.02 nA}. This conductance value might be slightly underestimated because Ih was activated by the commanding hyperpolarizing voltage step from -60 mV, where about 5% of Ih channels were already activated (see Fig. 6C).\n",
      "Context:  re slowly activated (n  28; Fig. 7A; also see Fig. 6C). By measuring the difference between the instantaneous and steady-state inward currents, the maximal Ih-mediated inward currents were obtained. The amplitudes of Ih currents exhibited a scattered distribution between 12.8 and 159.5 pA, with a mean value of 56.6 ^ 32.1 pA (Fig. 7B). This gave a maximal Ih conductance of 1.02 nS, assuming its reversal potential to be 244 mV {i.e. g  I/(Vm 2 Eh)  257 pA/ [2100 mV 2 (244 mV)]  1.02 nA}. This conductance value might be slightly underestimated because Ih was activated by the commanding hyperpolarizing voltage step from 2 60 mV, where about 5% of Ih channels were already activated (see Fig. 6C). The inward currents were correlated with the  instantaneous Ileak currents (Fig. 7C; r  0.649, P , 0.0005, ANOVA), suggesting that if an interneuron has a large Ileak conductance, it will also have a \n",
      "Values:  1.02\n",
      "Unit:  nA\n",
      " \n",
      "Number:  7\n",
      "Text:  The range of dendritic T-current conductance obtained was ϳ0.8 –1.4 mS/cm 2 (8 –14 pS/ ␮ m 2 )\n",
      "Context:  e soma. To compare the above dendritic T-current permeabilities with published values, they were converted into conductances by simulating the calcium current using the Nernst relation (Hille, 1992). The range of dendritic T-current conductance obtained was ϳ0.8 –1.4 mS/cm 2 (8 –14 pS/␮m 2). This estimated conductance range is slightly larger but close to the average T-current densities of 7–10 pS/␮m 2 measured in the dendrites of hippocampal pyramidal cells (Magee and Johnston, 1995).  \f",
      "D\n",
      "Values:    [0.8 - 1.4] \n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  8\n",
      "Text:  delayed rectifier potassium current\n",
      "Context:  e erratum of that publication (http://huguenard-lab. stanford.edu/pubs.php) using a maximum permeability of 1.0 ⫻ 10⫺4 cm/s. Finally, the calcium-activated potassium conductances IKCa and IKAHP were incorporated into the model by using parameters from Traub et al. (2003) as implemented by Lazarewicz and Traub in ModelDB (https://senselab.med.yale.edu/modeldb/, accession no. 20756) with a correction factor of 10⫺6 to convert their a\n",
      "Values:  0.002\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  9\n",
      "Text:  However, if I, was located only in the soma using the geometry of Figure lB, the density needed to match the same recordings was 0.1 mS/cm*.\n",
      "Context:  to be adjustedaccordingly: for a uniform density, the best fit was obtained with 0.045mS/cm’ (Fig. 6), independentlyof the particular details of the morphology chosento represent dissociatedRE cells. However, if I, was located only in the soma using the geometry of Figure lB, the density needed to match the same recordingswas 0.1 mS/cm*. The valuesof I, density from the dissociatedcell model were then introduced in the modelwith an intact cell morphology.Both a uniform density of 0.045 mS/cm’ (Fig. 7, left) and a somatic density of 0.\n",
      "Values:  3.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  10\n",
      "Text:  maximal conductance gNa = 0 1 S/cm2 . and gK = 0 1 S/cm2 . , and reversal potentials of ENa = 50 mV and EK = −100 mV.\n",
      "Context:  tential required the permeability PCa = 1.56 × 10−4 cm/s.  www.frontiersin.org  December 2008 | Volume 2 | Article 5 | 3  \f",
      "Zomorrodi et al.  Model of thalamocortical neuron  potential of VT = −52 mV, maximal conductance g Na = 0.1 S/cm 2 and g K = 0.1 S/cm 2, and reversal potentials of ENa = 50 mV and EK = −100 mV. This model has been shown to be adequate to model the repetitive firing within bursts of action potentials (Destexhe et al., 1996). Because of the nonlinear and far-from-equilibrium behavior of calciu\n",
      "Values:  0.1\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  11\n",
      "Text:  e Na ϩ and K ϩ currents responsible for fast action poten- tials, they were inserted in the soma, and their kinetics were taken from a model of hippocampal pyramidal cells (Traub and Miles, 1991), assum- ing a resting potential of V T ϭ Ϫ52 mV in their equations, maximal conductances of g ៮ Na ϭ 100 mS/cm 2 and g ៮ K ϭ 100 mS/cm 2 , and reversal potentials of E Na ϭ 50 mV and E K ϭ Ϫ100 mV\n",
      "Context:  t currents. Voltage-dependent conductances were modeled using a Hodgkin –Huxley type of kinetic model (Hodgkin and Huxley, 1952). Because no data exist to constrain the localization and kinetics of the Na ϩ and K ϩ currents responsible for fast action potentials, they were inserted in the soma, and their kinetics were taken from a model of hippocampal pyramidal cells (Traub and Miles, 1991), assuming a resting potential of VT ϭ Ϫ52 mV in their equations, maximal conductances of g៮ Na ϭ 100 mS/cm 2 and g៮ K ϭ 100 mS/cm 2, and reversal potentials of ENa ϭ 50 mV and EK ϭ Ϫ100 mV. This model was already shown to be adequate to model the repetitive firing within bursts of action potentials (Traub and Miles, 1991; Destexhe et al., 1996a,b). The kinetics of activation and inactivation of \n",
      "Values:  100.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  12\n",
      "Text:  Amplitude of this transient current, about one-third of the total unblocked current, had a peak of 205.4 +/- 49.1 pA (n = 3). This amplitude was somewhat greater than observed previously in dissociated cells [58]. This might be due to channels present in the slice preparation that had been removed or inactivated during cell dissociation, or could be a result of the temperature difference, due to the relatively large Q10 for permeability of this channel.\n",
      "Context:  in TC cells, with a voltage threshold of approximately ⫺ 58 mV. Time constants of activation and inactivation appeared to be consistent with previous descriptions of IT in other thalamic cells. 14,33 Amplitude of this transient current, about one-third of the total unblocked current, had a peak of 205.4 ^ 49.1 pA (n  3). This amplitude was somewhat greater than observed previously in dissociated cells. 58 This might be due to channels present in the slice preparation that had been removed or inactivated during cell dissociation, or could be a result of the temperature difference, due to the relatively large Q10 for permeability of this channel. While the persistent current was clearly evident in all six cells tested, its time-course varied considerably, showing a slow decay in two cases (e.g., Fig. 7A) and a more rapid decay in four other cases\n",
      "Values:  205.4 +/- 49.1  (n=3.0)\n",
      "Unit:  pA\n",
      " \n",
      "Number:  13\n",
      "Text:  3–15 nS with a patch electrode (our unpublished data; also see Ref. 9)\n",
      "Context:  scribed in other cell types, where values of 2–10 nS have generally been reported. In thalamocortical cells, in particular, a maximal Ih conductance of 10–40 nS was found with a sharp electrode 33 or 3–15 nS with a patch electrode (our unpublished data; also see Ref. 9). TRN cells, on the other hand, appear to have no Ih channels. 5,10 Despite its small maximal conductance, Ih in interneurons can produce a pronounced depolarizing sag with hyperpolarizing current cla\n",
      "Values:    [3.0 - 15.0] \n",
      "Unit:  nS\n",
      " \n",
      "Number:  14\n",
      "Text:  the calcium-activated potassium conductances IKCa and IKAHP were incorporated into the model by using parameters from Traub et al. (2003) as implemented by Lazarewicz and Traub in ModelDB (https://senselab.med.yale.edu/modeldb/, accession no. 20756) with a correction factor of 10\u00056 to convert their arbitrary units of calcium concentration to millimoles per liter. Maximum conductances of 1.0 \u0006 10\u00054 and 1.5 \u0006 10\u00055 S/cm2 were used for IKCa and IKAHP, respectively.\n",
      "Context:  use of pharmacology: a fast component sensitive to barium and a slow component sensitive to the drug ZD-7288 (Williams et al. 1997). The slow component is mediated by the hyperpolarization-activated cationic current Ih (see below), whereas an inwardly rectifying potassium current (IKir) has been assumed to underlie the fast component (Williams et al. 1997). Yet, Ikir has not been fully characterized in these cells, and its role is unknown. To isolate IKir, we initially used a protocol of hyperpolarizing pulses in whole cell voltage clamp and application of 50 ␮M Ba2⫹ (see METHODS) (Fig. 1A). The barium-sensitive component quickly reached steady state (␶ ⫽ 5.7 ⫾ 0.6 ms at ⫺114 mV; n ⫽ 9), whereas the remaining barium-insensitive component rose very slowly and did not reach steady state during the duration of the 1-s-long pulse protocol. The fast kinetics\n",
      "Values:  0.0001\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  15\n",
      "Text:   were adjusted to match the slope and the extrapolated reversal potential\n",
      "Context:  eady-state conductance of TC neurons at potentials positive to ⫺80 mV.  Vm(mV)  Finally, we performed a simulation including all the identified components, using the maximum conductance values established in the preceding sections, and compared it to the experimentally recorded I-V plots in the absence of pharmacological agents (Fig. 3D); this figure shows a rather precise correspondence between the simulation and the experimental data. This computational reconstructio\n",
      "Values:  1e-05\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  16\n",
      "Text:  current were chosen to be 500 and 100 pS/lm2, respectively, and the peak conductance density for the non-inactivating K+ current was chosen to be 5 pS/lm2.\n",
      "Context:  amics of synapses according to the type (excitatory or inhibitory) of the pre- and postsynaptic neuron: mean values of U, D, and F according to Markram and others (1998) and Gupta and others (2000).  current were chosen to be 500 and 100 pS/lm2, respectively, and the + peak conductance density for the non-inactivating K current was 2 chosen to be 5 pS/lm . The membrane area of the neuron was set to be 34 636 lm2 as in Destexhe and others (2001). For each simulation, the initial conditions of each neuron, that is, the membrane voltage at time t = 0, \n",
      "Values:  5.0\n",
      "Unit:  pS/um**2\n",
      " \n",
      "Number:  17\n",
      "Text:  when the difference in membrane surface area between the two types of cells (see above) is taken into account (1.1 pA/um^2 in interneurones, and 1.3 pA/um^2 in relay cells).\n",
      "Context:  e maximal amplitude of the whole-cell current is substantially smaller in size in local circuit neurones than that in relay neurones, the mean size of IT is very similar in the two types of LGN cells when the difference in membrane surface area between the two types of cells (see above) is taken into account (11 pA tm-2 in interneurones, and 1'3 pA /Lm-2 in relay cells). Very interestingly, interneurones were also found to possess a non-inactivating, L-type Ca2+ current very similar to that of relay cells (cf. Hernandez-Cruz & Pape, 1989), which (i) was activated thr\n",
      "Values:  1.1\n",
      "Unit:  pA/um^2\n",
      " \n",
      "Number:  18\n",
      "Text:  when the difference in membrane surface area between the two types of cells (see above) is taken into account (1.1 pA/um^2 in interneurones, and 1.3 pA/um^2 in relay cells).\n",
      "Context:  e maximal amplitude of the whole-cell current is substantially smaller in size in local circuit neurones than that in relay neurones, the mean size of IT is very similar in the two types of LGN cells when the difference in membrane surface area between the two types of cells (see above) is taken into account (11 pA tm-2 in interneurones, and 1'3 pA /Lm-2 in relay cells). Very interestingly, interneurones were also found to possess a non-inactivating, L-type Ca2+ current very similar to that of relay cells (cf. Hernandez-Cruz & Pape, 1989), which (i) was activated thr\n",
      "Values:  1.3\n",
      "Unit:  pA/um^2\n",
      " \n",
      "Number:  19\n",
      "Text:  the range of uniformly distributed gT densities capable of reproducing the voltage signals we recorded in vitro was relatively narrow, and the optimal value ( gT, 7 \u0011 10\t5 cm/s)\n",
      "Context:  ordings (Fig. 9B) while also reproducing intracellular ⌬[Ca2⫹] equivalent to those described previously (Fig. 10B; Crandall et al., 2010; Errington et al., 2010, 2012; Sieber et al., 2013). Moreover, the range of uniformly distributed gT densities capable of reproducing the voltage signals we recorded in vitro was relatively narrow, and the optimal value ( gT, 7 ⫻ 10 ⫺5 cm/s) was similar to that reported in a previous study (Destexhe et al., 1998; Fig. 9C). Thus, using these parameters, our TC neuron model could reproduce both the electrical signals and ⌬[Ca2⫹] observed \n",
      "Values:  7e-05\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  20\n",
      "Text:  rising to 8mS cm−2 in the proximal branches\n",
      "Context:  e soma to the proximal stems and then rapidly falling off by 40 µm from the soma. To take into account experimental measurements of dendritic and somatic T-current density, our model incorporates a conductance density of 2 mS cm−2 in the soma, rising to 8 mS cm−2 in the proximal branches and falling off to 1 mS cm−2 in the distal branches. Thus the present model has a mainly proximal dendritic CaT distribution. The production of rebound bu\n",
      "Values:  8.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  21\n",
      "Text:  When the difference in membrane surface area between the two types of cells is taken into account (see above), the maximal A-conductance is slightly (P< 0 01) higher in interneurones (9.6 pS/um2) compared with that in relay cells (8.0 pS/um2).\n",
      "Context:  interneurones compared with that in relay cells, and the process of inactivation (and removal from inactivation) of IA takes significantly longer to be completed in interneurones than in relay cells. When the difference in membrane surface area between the two types of cells is taken into account (see above), the maximal A-conductance is slightly (P< 0 01) higher in interneurones (9-6 pS ,#m-2) compared with that in relay cells (8-0 pS /mM-2).  A  Relay cell (e)  415  Interactions between IT and IA The voltage-clamp analysis revealed many similarities between IA and IT in terms of their time courses and voltage dependencies. There are,\n",
      "Values:  9.6\n",
      "Unit:  pS/um**2\n",
      " \n",
      "Number:  22\n",
      "Text:  Only the density of T-channels had to be adjusted accordingly: for a uniform density, the best fit was ob- tained with 0.045 mS/cm’\n",
      "Context:  ics of the T-current wasneededto match the voltage-clampdata usingthe modelof the dissociatedRE cell  Destexhe  et al. . Dendritically  Generated  Bursts  in Thalamic  RE Neurons  shown in Figure 1B. Only the density of T-channelshad to be adjustedaccordingly: for a uniform density, the best fit was obtained with 0.045mS/cm’ (Fig. 6), independentlyof the particular details of the morphology chosento represent dissociatedRE cells. However, if I, was located only in the soma using the geometry of Figure lB, the density needed t\n",
      "Values:  0.3\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  23\n",
      "Text:  The gNa had conductance of 50 mS/cm2 and reversal potential of 50 mV\n",
      "Context:  ntial of ⫺20 mV. The gKIR and the fast voltage-gated potassium channel ( gKf) had conductances of 20 ␮s/ cm 2 and 50 mS/cm 2, respectively. Both potassium channels had reversal potentials of ⫺100 mV. The gNa had conductance of 50 mS/cm 2 and reversal potential of 50 mV. Throughout this manuscript, when referring to the ability of gT to provide current, we use the term “conductance” rather than “permeability.” Whereas the model is in actuality based on permeability\n",
      "Values:  50.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  24\n",
      "Text:  The gKIR and the fast voltage-gated potassium channel ( gKf) had conductances of 20 \u0002s/ cm2 and 50 mS/cm2, respectively. Both potassium channels had reversal potentials of 100 mV.\n",
      "Context:  of 150 ␮s/cm 2. T-type Ca2⫹ channels ( gT) were modeled with a reversal potential of 120 mV and a permeability of 0.7 ␮m/s. The gCAN had a conductance of 250 ␮S/cm 2 and reversal potential of ⫺20 mV. The gKIR and the fast voltage-gated potassium channel ( gKf) had conductances of 20 ␮s/ cm 2 and 50 mS/cm 2, respectively. Both potassium channels had reversal potentials of ⫺100 mV. The gNa had conductance of 50 mS/cm 2 and reversal potential of 50 mV. Throughout this manuscript, when referring to the ability of gT to provide current, we use the term “conductance” rather than\n",
      "Values:  20.0\n",
      "Unit:  uS/cm^2\n",
      " \n",
      "Number:  25\n",
      "Text:  the calcium-activated potassium conductances IKCa and IKAHP were incorporated into the model by using parameters from Traub et al. (2003) as implemented by Lazarewicz and Traub in ModelDB (https://senselab.med.yale.edu/modeldb/, accession no. 20756) with a correction factor of 10\u00056 to convert their arbitrary units of calcium concentration to millimoles per liter. Maximum conductances of 1.0 \u0006 10\u00054 and 1.5 \u0006 10\u00055 S/cm2 were used for IKCa and IKAHP, respectively.\n",
      "Context:  use of pharmacology: a fast component sensitive to barium and a slow component sensitive to the drug ZD-7288 (Williams et al. 1997). The slow component is mediated by the hyperpolarization-activated cationic current Ih (see below), whereas an inwardly rectifying potassium current (IKir) has been assumed to underlie the fast component (Williams et al. 1997). Yet, Ikir has not been fully characterized in these cells, and its role is unknown. To isolate IKir, we initially used a protocol of hyperpolarizing pulses in whole cell voltage clamp and application of 50 ␮M Ba2⫹ (see METHODS) (Fig. 1A). The barium-sensitive component quickly reached steady state (␶ ⫽ 5.7 ⫾ 0.6 ms at ⫺114 mV; n ⫽ 9), whereas the remaining barium-insensitive component rose very slowly and did not reach steady state during the duration of the 1-s-long pulse protocol. The fast kinetics\n",
      "Values:  1.5e-05\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  26\n",
      "Text:  the mean value of I h conductance (0.13 mS/cm 2 )\n",
      "Context:  e product  \f",
      "454  J. J. Zhu et al.  Fig. 8. Simulation of the I–V relationship in a model thalamic interneuron. (A) Voltage-clamp simulations of Ihmediated slow inward currents in the model cell using the mean value of Ih conductance (0.13 mS/cm 2). The holding potential between the trials was 260 mV. (B) Current-clamp simulation of Ih-mediated inward rectification in the same model cell. (C) Summary I–V curves, derived from current-clamp simulat\n",
      "Values:  0.13\n",
      "Unit:  mS/cm**2\n",
      " \n",
      "Number:  27\n",
      "Text:  To take into account experimental measurements of dendritic and somatic T-current density, our model incorporates a conductance density of 2 mS cm−2\n",
      "Context:  These groups patched relay cell dendrites to about 50 µm from the soma. Williams & Stuart (2000) found CaT conductance density rising approximately 2-fold from the soma to the proximal stems and then rapidly falling off by 40 µm from the soma. To take into account experimental measurements of dendritic and somatic T-current density, our model incorporates a conductance density of 2 mS cm−2 in the soma, rising to 8 mS cm−2 in the proximal branches and falling off to 1 mS cm−2 in the distal branches. Thus the present model has a mainly proximal\n",
      "Values:  2.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  28\n",
      "Text:  INaleak (3.0 \u0006 10\u00056 S/cm2)\n",
      "Context:  rents contribute to the steady-state conductance of TC neurons at potentials positive to ⫺80 mV.  Vm(mV)  Finally, we performed a simulation including all the identified components, using the maximum conductance values established in the preceding sections, and compared it to the experimentally recorded I-V plots in the absence of pharmacological agents (Fig. 3D); this figure shows a rather precise correspondence between t\n",
      "Values:  3e-06\n",
      "Unit:  S/cm**2\n",
      " \n",
      "Number:  29\n",
      "Text:  The maximum conductance for IA ( \u0002gA 5.5 \u0006 10\u00053 S/cm2)\n",
      "Context:  ltagedependent contributions to the total steady-state current (black). The main determinants of the RMP (the reversal potential of the total I-V curve) in these neurons, determined as fractions of the total current, are the leak conductances gKleak and gNaleak (36.7% and 24.5% of the total conductance, respectively). The other contributing conductances (in decreasing order) are gT (11.2%), gA (10.7%), gNaP (7.5%), gh (5.8%), and gKir (3.5%) (Fig. 4B).\n",
      "Values:  0.0055\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  30\n",
      "Text:  lthough we observed a typical T-type Ca2` current in more than 80% of our sample of interneurones (n = 34), it was significantly smaller in size (mean maximal amplitude, 109 +/- 46 pA; n = 11) than that in relay cells (289 +/- 131 pA; n = 16)\n",
      "Context:   & Pape, 1989), decreased in size after equimolar substitution of extracellular Ca2\" by barium ions (Ba2+), and was readily blocked by addition of 50 /SM Ni2` to the bathing medium (data not shown). Although we observed a typical T-type Ca2` current in more than 80% of our sample of interneurones (n = 34), it was significantly smaller in size (mean maximal amplitude, 109 + 46 pA; n = 11) than that in relay cells (289 + 131 pA; n = 16), and the steady-state voltage dependence of IT was different in the two types of LGN neurones. Figure 5 illustrates experiments in which activation and inactivation properties of IT were analysed more qu\n",
      "Values:  109.0 +/- 46.0  (n=11.0)\n",
      "Unit:  pA\n",
      " \n",
      "Number:  31\n",
      "Text:  When the difference in membrane surface area between the two types of cells is taken into account (see above), the maximal A-conductance is slightly (P< 0 01) higher in interneurones (9.6 pS/um2) compared with that in relay cells (8.0 pS/um2).\n",
      "Context:  interneurones compared with that in relay cells, and the process of inactivation (and removal from inactivation) of IA takes significantly longer to be completed in interneurones than in relay cells. When the difference in membrane surface area between the two types of cells is taken into account (see above), the maximal A-conductance is slightly (P< 0 01) higher in interneurones (9-6 pS ,#m-2) compared with that in relay cells (8-0 pS /mM-2).  A  Relay cell (e)  415  Interactions between IT and IA The voltage-clamp analysis revealed many similarities between IA and IT in terms of their time courses and voltage dependencies. There are,\n",
      "Values:  8.0\n",
      "Unit:  pS/um**2\n",
      " \n",
      "Number:  32\n",
      "Text:  maximal conductance gNa = 0 1 S/cm2 . and gK = 0 1 S/cm2 . , and reversal potentials of ENa = 50 mV and EK = −100 mV.\n",
      "Context:  tential required the permeability PCa = 1.56 × 10−4 cm/s.  www.frontiersin.org  December 2008 | Volume 2 | Article 5 | 3  \f",
      "Zomorrodi et al.  Model of thalamocortical neuron  potential of VT = −52 mV, maximal conductance g Na = 0.1 S/cm 2 and g K = 0.1 S/cm 2, and reversal potentials of ENa = 50 mV and EK = −100 mV. This model has been shown to be adequate to model the repetitive firing within bursts of action potentials (Destexhe et al., 1996). Because of the nonlinear and far-from-equilibrium behavior of calciu\n",
      "Values:  0.1\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  33\n",
      "Text:  The gKIR and the fast voltage-gated potassium channel ( gKf) had conductances of 20 \u0002s/ cm2 and 50 mS/cm2, respectively. Both potassium channels had reversal potentials of 100 mV.\n",
      "Context:  of 150 ␮s/cm 2. T-type Ca2⫹ channels ( gT) were modeled with a reversal potential of 120 mV and a permeability of 0.7 ␮m/s. The gCAN had a conductance of 250 ␮S/cm 2 and reversal potential of ⫺20 mV. The gKIR and the fast voltage-gated potassium channel ( gKf) had conductances of 20 ␮s/ cm 2 and 50 mS/cm 2, respectively. Both potassium channels had reversal potentials of ⫺100 mV. The gNa had conductance of 50 mS/cm 2 and reversal potential of 50 mV. Throughout this manuscript, when referring to the ability of gT to provide current, we use the term “conductance” rather than\n",
      "Values:  50.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  34\n",
      "Text:  Assuming the single channel con- ductance of T-type channel as 7.5 pS\n",
      "Context:  \n",
      "Values:  7.5\n",
      "Unit:  pS\n",
      " \n",
      "Number:  35\n",
      "Text:  had a reversal potential of 45 mV and peak conductance of 150 \u0002s/cm2.\n",
      "Context:  ysiological data, to account for their conductance at rest. Thus, our final model comprised the following: gLEAK was modeled with a reversal potential of ⫺79 mV and conductance of 150 ␮s/cm 2, and gH had a reversal potential of ⫺45 mV and peak conductance of 150 ␮s/cm 2. T-type Ca2⫹ channels ( gT) were modeled with a reversal potential of 120 mV and a permeability of 0.7 ␮m/s. The gCAN had a conductance of 250 ␮S/cm 2 and reversal potential of ⫺20 mV. The gKIR and \n",
      "Values:  150.0\n",
      "Unit:  uS/cm^2\n",
      " \n",
      "Number:  36\n",
      "Text:  Fast transiente sodium current \n",
      "Context:  1992) with the corrections included in the erratum of that publication (http://huguenard-lab. stanford.edu/pubs.php) using a maximum permeability of 1.0 ⫻ 10⫺4 cm/s. Finally, the calcium-activated potassium conductances IKCa and IKAHP were incorporated into the model by using parameters from Traub et al. (2003) as implemented by Lazarewicz and Traub in ModelDB (https://senselab.med.yale.edu/modeldb/, accession no. 20756) with a\n",
      "Values:  0.01\n",
      "Unit:  S/cm^2\n",
      " \n",
      "Number:  37\n",
      "Text:  our final model comprised the following: gLEAK was modeled with a reversal potential of 79mV and conductance of 150\u0002s/cm2,\n",
      "Context:  ur electrophysiological data. Once the necessary channels were inserted into the model, we recalculated gLEAK, by fitting to electrophysiological data, to account for their conductance at rest. Thus, our final model comprised the following: gLEAK was modeled with a reversal potential of ⫺79 mV and conductance of 150 ␮s/cm 2, and gH had a reversal potential of ⫺45 mV and peak conductance of 150 ␮s/cm 2. T-type Ca2⫹ channels ( gT) were modeled with a reversal potential of 120 mV and a permeability of 0.7 ␮m/s. The gCAN\n",
      "Values:  150.0\n",
      "Unit:  uS/cm^2\n",
      " \n",
      "Number:  38\n",
      "Text:  The Na+ and K+ currents responsible for fast action potentials were inserted in the soma, and their kinetics was taken from a model of hippocampal pyra- midal cells (Traub and Miles, 1991), assuming a resting potential of -67 mV in their equations, maximal conductances of & = 100 mS/cm’ and & = 80 mS/cm*, and reversal potentials of E,, = 50 mV and E, = - 100 mV\n",
      "Context:  e, because the model included only a subset of the currents present in these cells. Voltage-dependent conductances were modeled using a HodgkinHuxley type of kinetic model (Hodgkin and Huxley, 1952). The Na+ and K+ currents responsible for fast action potentials were inserted in the soma, and their kinetics was taken from a model of hippocampal pyramidal cells (Traub and Miles, 1991), assuming a resting potential of -67 mV in their equations, maximal conductances of & = 100 mS/cm’ and & = 80 mS/cm*, and reversal potentials of E,, = 50 mV and E, = - 100 mV. The kinetics of activation and inactivation of I, in RE cells, as well as the activation curves of I,, obtained from voltage-clamp recordings in acutely dissociated RE cells (Huguenard and Prince, 199\n",
      "Values:  100.0\n",
      "Unit:  mS/cm^2\n",
      " \n",
      "Number:  39\n",
      "Text:  In thalamocortical cells, in particular, a maximal I h conductance of 10–40 nS was found with a sharp electrode 33\n",
      "Context:  onditions of lower input resistance. The maximal conductance of Ih in interneurons appears to be smaller than that described in other cell types, where values of 2–10 nS have generally been reported. In thalamocortical cells, in particular, a maximal Ih conductance of 10–40 nS was found with a sharp electrode 33 or 3–15 nS with a patch electrode (our unpublished data; also see Ref. 9). TRN cells, on the other hand, appear to have no Ih channels. 5,10 Despite its small maximal conductance, Ih in interneurons c\n",
      "Values:    [10.0 - 40.0] \n",
      "Unit:  nS\n",
      " \n",
      "Number:  40\n",
      "Text:  current were chosen to be 500 and 100 pS/lm2, respectively, and the peak conductance density for the non-inactivating K+ current was chosen to be 5 pS/lm2.\n",
      "Context:  amics of synapses according to the type (excitatory or inhibitory) of the pre- and postsynaptic neuron: mean values of U, D, and F according to Markram and others (1998) and Gupta and others (2000).  current were chosen to be 500 and 100 pS/lm2, respectively, and the + peak conductance density for the non-inactivating K current was 2 chosen to be 5 pS/lm . The membrane area of the neuron was set to be 34 636 lm2 as in Destexhe and others (2001). For each simulation, the initial conditions of each neuron, that is, the membrane voltage at time t = 0, \n",
      "Values:  500.0\n",
      "Unit:  pS/um**2\n",
      " \n",
      "PARAMETER NAME: neuron_density\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Number:  1\n",
      "Text:  The cellular density in Rt was 700 neurons/\\nmmz in the rat\n",
      "Context:  ar differences similar to those previously described [2,3,38]. The GABA/non-GABA ratio was close to 0.8 for both species (Fig. 5); the Rt/thalamus ratio was 10.12% in the rat and 10.31% in the mouse. The cellular density in Rt was 700 neurons/ mmz in the rat and 1087 neurons/mmz in the mouse, closer to that of the bat than to that of the rat (Figs. 6 and 7B ). Guinea Pig The main concentration of GABA-ir cells was found within Rt, where all the neurons of\n",
      "Values:  700.0\n",
      "Unit:  1/mm^2\n",
      " \n",
      "Number:  2\n",
      "Text:  As estimated with StereoInvestigator, the neuronal density was the highest in PF (51,465 +/- 3,577 neurons/mm^3 ), followed by DLG (40,069 +/- 1,540) and Rt (31,884 +/- 3,351; PF > DLG, P < 0.05; PF > Rt, P < 0.01).\n",
      "Context:  and 0.76 Ϯ 0.11 mm3 (PF Ͻ DLG and Rt, P Ͻ 0.01). In cresyl violet-stained sections, the neuronal cell bodies in DLG, PF, and Rt were of comparable size and shape, averaging 15.8 Ϯ 0.7 ␮m in diameter. As estimated with StereoInvestigator, the neuronal density was the highest in PF (51,465 Ϯ 3,577 neurons/mm3), followed by DLG (40,069 Ϯ 1,540) and Rt (31,884 Ϯ 3,351; PF Ͼ DLG, P Ͻ 0.05; PF Ͼ Rt, P Ͻ 0.01). The number of ChATimmunostained varicosities per cubic micrometer of tissue (i.e., the regional density of ChAT innervation) was also the highest in PF (2.1 Ϯ 0.2 million ChAT-immunostained axon varicosities\n",
      "Values:  40069.0 +/- 1540.0  (n=5.0)\n",
      "Unit:  1/mm^3\n",
      " \n",
      "Number:  3\n",
      "Text:  The total volume of this part of vibrissal thalamus (i.e., convex hull around 24 barreloids; Materials and Methods) was 0.19 ± 0.03 mm 3 , resulting in an average neuron density across the entire VPM of 52,494 ± 5,082 mm −3 . Densities in VPM within and above/below barreloids (51,507 ± 4,422 and 54,440 ± 6,559 mm −3 , respectively) were larger compared with the sur- rounding thalamic nuclei, with 49,680 ± 1,097 and 41,477 ± 3,612mm −3 in nucleus reticularis (RT) and posterior medial nucleus (POm), respectively. \n",
      "Context:  vibrissal thalamus was 9,963 ± 718. As for the vibrissal cortex, the variability in neuron numbers across animals was small (7.2%). All neurons in VPM thalamus were excitatory (i.e., GAD67 negative). The total volume of this part of vibrissal thalamus (i.e., convex hull around 24 barreloids; Materials and Methods) was 0.19 ± 0.03 mm3, resulting in an average neuron density across the entire VPM of 52,494 ± 5,082 mm−3. Densities in VPM within and above/below barreloids (51,507 ± 4,422 and 54,440 ± 6,559 mm−3, respectively) were larger compared with the surrounding thalamic nuclei, with 49,680 ± 1,097 and 41,477 ± 3,612 Meyer et al.  Whisker-Speciﬁc Horizontal Organization. We calculated the direction of the gradient within the horizontal plane of the vibrissal cortex (Fig. 3, arrows) for the volume, neuron density, neuron number, and fraction of inhibitory neurons per barrel column, respectively. The hori\n",
      "Values:  52494.0 +/- 5082.0  (n=3.0)\n",
      "Unit:  1/mm^3\n",
      " \n",
      "Number:  4\n",
      "Text:  The total volume of this part of vibrissal thalamus (i.e., convex hull around 24 barreloids; Materials and Methods) was 0.19 ± 0.03 mm 3 , resulting in an average neuron density across the entire VPM of 52,494 ± 5,082 mm −3 . Densities in VPM within and above/below barreloids (51,507 ± 4,422 and 54,440 ± 6,559 mm −3 , respectively) were larger compared with the sur- rounding thalamic nuclei, with 49,680 ± 1,097 and 41,477 ± 3,612mm −3 in nucleus reticularis (RT) and posterior medial nucleus (POm), respectively. \n",
      "Context:  vibrissal thalamus was 9,963 ± 718. As for the vibrissal cortex, the variability in neuron numbers across animals was small (7.2%). All neurons in VPM thalamus were excitatory (i.e., GAD67 negative). The total volume of this part of vibrissal thalamus (i.e., convex hull around 24 barreloids; Materials and Methods) was 0.19 ± 0.03 mm3, resulting in an average neuron density across the entire VPM of 52,494 ± 5,082 mm−3. Densities in VPM within and above/below barreloids (51,507 ± 4,422 and 54,440 ± 6,559 mm−3, respectively) were larger compared with the surrounding thalamic nuclei, with 49,680 ± 1,097 and 41,477 ± 3,612 Meyer et al.  Whisker-Speciﬁc Horizontal Organization. We calculated the direction of the gradient within the horizontal plane of the vibrissal cortex (Fig. 3, arrows) for the volume, neuron density, neuron number, and fraction of inhibitory neurons per barrel column, respectively. The hori\n",
      "Values:  41477.0 +/- 3612.0  (n=3.0)\n",
      "Unit:  1/mm^3\n",
      " \n",
      "Number:  5\n",
      "Text:  From fixed tissue and 70 μm thick coronal sections through the middle of dLGN, the average density of GFP positive interneurons in dLGN was around one to two cells per 2500 μm^2 .\n",
      "Context:   strain, GFP is not expressed in GABAergic cells of the thalamic reticular nucleus, therefore providing unambiguous visualization and access to intrinsic interneurons and their processes in dLGN [9]. From fixed tissue and 70 μm thick coronal sections through the middle of dLGN, the average density of GFP positive interneurons in dLGN was around one to two cells per 2500 μm2. At all ages examined (P7, P9, and P26), these cells were evenly distributed throughout dLGN (Figure 1B) and showed no preference for either the monocular or binocular regions of dLGN (one-way analysis \n",
      "Values:    [0.0004 - 0.0008] (n=3.0)\n",
      "Unit:  1/um^2\n",
      " \n",
      "Number:  6\n",
      "Text:  As estimated with StereoInvestigator, the neuronal density was the highest in PF (51,465 +/- 3,577 neurons/mm^3 ), followed by DLG (40,069 +/- 1,540) and Rt (31,884 +/- 3,351; PF > DLG, P < 0.05; PF > Rt, P < 0.01).\n",
      "Context:  and 0.76 Ϯ 0.11 mm3 (PF Ͻ DLG and Rt, P Ͻ 0.01). In cresyl violet-stained sections, the neuronal cell bodies in DLG, PF, and Rt were of comparable size and shape, averaging 15.8 Ϯ 0.7 ␮m in diameter. As estimated with StereoInvestigator, the neuronal density was the highest in PF (51,465 Ϯ 3,577 neurons/mm3), followed by DLG (40,069 Ϯ 1,540) and Rt (31,884 Ϯ 3,351; PF Ͼ DLG, P Ͻ 0.05; PF Ͼ Rt, P Ͻ 0.01). The number of ChATimmunostained varicosities per cubic micrometer of tissue (i.e., the regional density of ChAT innervation) was also the highest in PF (2.1 Ϯ 0.2 million ChAT-immunostained axon varicosities\n",
      "Values:  31884.0 +/- 3351.0  (n=5.0)\n",
      "Unit:  1/mm^3\n",
      " \n",
      "Number:  7\n",
      "Text:  overall density of neurons in the dLGN of 5,434±326 cells/mm2\n",
      "Context:  sion of calbindin-D28K (CB), calretinin (CR) and parvalbumin (PV) were performed on dLGN slices (60 lm thick sections from 14to 21-day-old rats). Alternate sections were Nissl-stained and revealed an overall density of neurons in the dLGN of 5,434±326 cells/mm2 (measured in six slices from three animals). Western blot analysis of dLGN tissue using a CBspeciﬁc antibody revealed a band of 28 kDa present throughout postnatal development (Fig. 3A). In dLGN slic\n",
      "Values:  5434.0 +/- 326.0  (n=6.0)\n",
      "Unit:  1/mm^2\n",
      " \n",
      "Number:  8\n",
      "Text:  As estimated with StereoInvestigator, the neuronal density was the highest in PF (51,465 +/- 3,577 neurons/mm^3 ), followed by DLG (40,069 +/- 1,540) and Rt (31,884 +/- 3,351; PF > DLG, P < 0.05; PF > Rt, P < 0.01).\n",
      "Context:  and 0.76 Ϯ 0.11 mm3 (PF Ͻ DLG and Rt, P Ͻ 0.01). In cresyl violet-stained sections, the neuronal cell bodies in DLG, PF, and Rt were of comparable size and shape, averaging 15.8 Ϯ 0.7 ␮m in diameter. As estimated with StereoInvestigator, the neuronal density was the highest in PF (51,465 Ϯ 3,577 neurons/mm3), followed by DLG (40,069 Ϯ 1,540) and Rt (31,884 Ϯ 3,351; PF Ͼ DLG, P Ͻ 0.05; PF Ͼ Rt, P Ͻ 0.01). The number of ChATimmunostained varicosities per cubic micrometer of tissue (i.e., the regional density of ChAT innervation) was also the highest in PF (2.1 Ϯ 0.2 million ChAT-immunostained axon varicosities\n",
      "Values:  51465.0 +/- 3577.0  (n=5.0)\n",
      "Unit:  1/mm^3\n",
      " \n",
      "Number:  9\n",
      "Text:  The density of neurons in the middle region of the RT was 103.0 +/- 8.6 per 100 grid-field, corresponding to a normalized density of 17.8 +/- 1.5 X 10^3 neurons per mm^3.\n",
      "Context:  fibers coursing into and out of the internal capsule. Within these rays all types of RT neurons could be distinguished. All RT neurons label positively with antisera to GAD and parvalbumin (Fig. 2A). The density of neurons in the middle region of the RT was 103.0 + 8.6 per 100 grid-field, corresponding to a normalized density of 17.8 + 1.5 X 103 neurons per mm 3. Analysis of the mediolateral distribution of neurons in the middle RT revealed significant differences among the three subregions ( P < 0.001; 1-way ANOVA), characterized by densities in the medial and ce\n",
      "Values:  17800.0 +/- 1500.0  (n=8.0)\n",
      "Unit:  1/mm^3\n",
      " \n",
      "Number:  10\n",
      "Text:  The total volume of this part of vibrissal thalamus (i.e., convex hull around 24 barreloids; Materials and Methods) was 0.19 ± 0.03 mm 3 , resulting in an average neuron density across the entire VPM of 52,494 ± 5,082 mm −3 . Densities in VPM within and above/below barreloids (51,507 ± 4,422 and 54,440 ± 6,559 mm −3 , respectively) were larger compared with the sur- rounding thalamic nuclei, with 49,680 ± 1,097 and 41,477 ± 3,612mm −3 in nucleus reticularis (RT) and posterior medial nucleus (POm), respectively. \n",
      "Context:  vibrissal thalamus was 9,963 ± 718. As for the vibrissal cortex, the variability in neuron numbers across animals was small (7.2%). All neurons in VPM thalamus were excitatory (i.e., GAD67 negative). The total volume of this part of vibrissal thalamus (i.e., convex hull around 24 barreloids; Materials and Methods) was 0.19 ± 0.03 mm3, resulting in an average neuron density across the entire VPM of 52,494 ± 5,082 mm−3. Densities in VPM within and above/below barreloids (51,507 ± 4,422 and 54,440 ± 6,559 mm−3, respectively) were larger compared with the surrounding thalamic nuclei, with 49,680 ± 1,097 and 41,477 ± 3,612 Meyer et al.  Whisker-Speciﬁc Horizontal Organization. We calculated the direction of the gradient within the horizontal plane of the vibrissal cortex (Fig. 3, arrows) for the volume, neuron density, neuron number, and fraction of inhibitory neurons per barrel column, respectively. The hori\n",
      "Values:  49680.0 +/- 1097.0  (n=3.0)\n",
      "Unit:  1/mm^3\n",
      " \n",
      "PARAMETER NAME: neuron_number\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Number:  1\n",
      "Text:  No changes in the total number of nRt neurons were found (mean +/- SEM); GAERS = (30.5 +/- 2.4)*10^3 and controls = (31.7 +/- 2.0)*10^3\n",
      "Context:  d using optical fractionator in the CAST-Grid system (Olympus, DK). The total number of neocortical neurons was then calculated using the formulae: Q- \" E P '7\" ap.int Ntotal m. Udisector  3. Results No changes in the total number of nRt neurons were found (mean + SEM); GAERS = (30.5 + 2.4)l 0 3 and controls = (31.7 +_ 2.0). l0 s (Fig. la).  The number of neocor~ica~ neurons in comro[animals was (19.5 + 0.9)- l0 ~' (mean + SEM). in the GAE~,~S a non-significam 6.5% reduction to {!8,2 _!: 0.4). ~0 ~ was tbund (Fig. ~b).  4. Conclu\n",
      "Values:  31700.0 +/- 2000.0  (n=7.0)\n",
      "Unit:  dimensionless\n",
      " \n",
      "Number:  2\n",
      "Text:  Control animals had 41 000 ± 1800 (range 32 800– 48 800) PARV-ir neurons in the RT.\n",
      "Context:  ucleus as compared to that in controls (Fig. 3). Therefore, we performed an unbiased quantitative estimation of the PARV-ir neuronal numbers after TBI using the optical fractionator method (Fig. 4C). Control animals had 41 000 ± 1800 (range 32 800– 48 800) PARV-ir neurons in the RT. The number of PARV-ir neurons in the ipsilateral RT in rats with TBI was 64% of that in controls (p < 0.001) (Fig. 4C) and 79% of that contralaterally (p < 0.05) (Fig. 4C). Contralaterally, the numbe\n",
      "Values:  41000.0 +/- 1800.0 [32800.0 - 48800.0] (n=9.0)\n",
      "Unit:  dimensionless\n",
      " \n",
      "Number:  3\n",
      "Text:  The number of neocorrtical neurons in control animals was (19.5 +/- 0.9)*10^6 (mean +/- SEM).\n",
      "Context:  ae: Q- \" E P '7\" ap.int Ntotal m. Udisector  3. Results No changes in the total number of nRt neurons were found (mean + SEM); GAERS = (30.5 + 2.4)l 0 3 and controls = (31.7 +_ 2.0). l0 s (Fig. la).  The number of neocor~ica~ neurons in comro[animals was (19.5 + 0.9)- l0 ~' (mean + SEM). in the GAE~,~S a non-significam 6.5% reduction to {!8,2 _!: 0.4). ~0 ~ was tbund (Fig. ~b).  4. Conclusion Loss of neurons has been described in other models of epilepsy [5,7,15]. Repetitive generalized s\n",
      "Values:  19500000.0 +/- 900000.0  (n=7.0)\n",
      "Unit:  dimensionless\n",
      " \n",
      "Number:  4\n",
      "Text:  According to stereological determinations, the number of PV-IR neurons in the RTN in a young adult rat was 14,700\n",
      "Context:  the profiles of PV-immunonegative structures (nuclei + dendritic bulbs): that of neuronal nuclei varied between 5 mm2 and 84 mm2, while those of the dendritic bulbs varied between 68 mm2 and 101 mm2. According to stereological determinations, the number of PV-IR neurons in the RTN in a young adult rat was 14,700, while that of PVIR calyciform endings was 2040. In several cases it could be observed that perikarya of PVIR nerve cells were directly continuous with calyciform terminals. In such cases, the perika\n",
      "Values:  14700.0\n",
      "Unit:  dimensionless\n",
      " \n",
      "PARAMETER NAME: age\n",
      "Number:  1\n",
      "Text:  Experiments were performed in brain slices prepared from postnatal (P) 6- to 36-day-old rats, as previously described (Zhu & Lo, 1999; Zhu, 2000).\n",
      "Context:  oma are given the 1st order. Then, after each new bifurcation, the branch order is incremented by one and the two daughter branches are given the same order. Chemicals were purchased from Sigma-RBI.  Experiments were performed in brain slices prepared from postnatal (P) 6- to 36-day-old rats, as previously described (Zhu & Lo, 1999; Zhu, 2000). All procedures used conformed with the guidelines, and the approval, of the Norwegian Animal Research Authority and the University of Virginia Animal Care and Use Committee. In brief, the rats were d\n",
      "Values:    [6.0 - 36.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  2\n",
      "Text:  Sprague Dawley rats [postnatal day 12 (P12) to P15]\n",
      "Context:  g electrotonic coupling.  Materials and Methods In vitro slice preparation. All experiments were performed according to protocols approved by the Stanford Institutional Animal Care and Use Committee. Sprague Dawley rats [postnatal day 12 (P12) to P15] were anesthetized with pentobarbital (50 mg/kg) and decapitated, and the brains were rapidly removed and immersed in an ice-cold (4°C) slicing solution containing the following (in mM): 234 sucrose, \n",
      "Values:    [12.0 - 15.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  3\n",
      "this study.wo females at 7 months of age, were used for\n",
      "Context:  ed at 4\u0001 C for 4 weeks prior to Nissl staining with thionin. All other series were collected in tissue collection solution and kept at \u000270\u0001 C until further processing.  Rats  Histological processing  Four Sprague-Dawley rats, Rattus norvegicus, two males and two females at 7 months of age, were used for this study. All rats were retired breeders obtained from a commercial supplier (Charles River, San Diego, CA). Animals were perfused, as described below, within 24 hours  The procedure for Nissl-stained sections\n",
      "Values:  7.0\n",
      "Unit:  months\n",
      " \n",
      "Number:  4\n",
      "Text:  Experiments were performed in somatosensory neocortical slices from P2—56 Wistar rats.\n",
      "Context:  s of layer 5 pyramidal cells in the rat neocortex matured in the first 6 postnatal weeks. This study has previously been presented in abstract form (Zhu & Sakmann, 1997).  Slice preparation  METHODS  Experiments were performed in somatosensory neocortical slices from P2—56 Wistar rats. The brain slices were prepared as previously described (Zhu & Lo, 1999). All animal procedures were carried out according to the animal welfare guidelines of the Max Planck Society. In brief, the rat\n",
      "Values:    [2.0 - 56.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  5\n",
      "Text:  Experiments were performed in thalamic slices from four- to 10-week-old Sprague–Dawley rats (100–300 g; Harlan Sprague Dawley).\n",
      "Context:  00 V cm, respectively. The model cell was equipped with a Hodgkin–Huxley style conductance (Ih) and a leak conductance (Ileak). Thus, the model can be described by the following equation:  Physiology Experiments were performed in thalamic slices from four- to 10-week-old Sprague–Dawley rats (100–300 g; Harlan Sprague Dawley). All efforts were made to minimize suffering and the number of animals used. The rats were deeply anesthetized by halothane and decapitated. The brain was quickly removed into cold (6–88C) physiologic\n",
      "Values:    [4.0 - 10.0] \n",
      "Unit:  week\n",
      " \n",
      "Number:  6\n",
      "Text:  2- to 25-day-old\n",
      "Context:   VB in isolation reacted positively for the presence of GAD (not shown), and these were easily distinguished from the larger relay neurones. All experiments were carried out using cells isolated from 2- to 25-day-old Wistar rats. Dissociation methods were modified from those of Kay & Wong (1986). Pregnant rats were obtained at 18 days gestation from Simonsen Laboratories (Gilroy, CA, USA) and were housed in our a\n",
      "Values:    [2.0 - 25.0] \n",
      "Unit:  d\n",
      " \n",
      "Number:  7\n",
      "Text:  Sprague-Dawley rats (postnatal days 7-12)\n",
      "Context:   in a sustainedreleaseof GABA onto TCs. Portions  of these results have been published  in preliminary  form (Huguenard and Prince, 1991). Materials and Methods Tissuesliceandisolatedcellpreparation. Sprague-Dawley rats (postnatal days 7-12) were deeply anesthetized with pentobarbital(50 mg/kg) and decapitated, and the brain was rapidly removed and placed in chilled oxygenated Ringer’s solution. Coronal slices with a thickness of 400 pm \n",
      "Values:    [7.0 - 12.0] \n",
      "Unit:  d\n",
      " \n",
      "Number:  8\n",
      "Text:  The overall density of interneurons was determined by counting GFP positive cells within the boundaries of dLGN. These measurements were obtained from 2 to 3 sections corresponding to the middle of dLGN (n = 3 mice; P7, P9, and P26).\n",
      "Context:  bad, CA, USA) dissolved in PBS with 0.1% Triton X-100. Slices were washed with PBS and then mounted with ProLong Gold antifade reagent (Invitrogen). Cell density measurements of interneurons in dLGN  The overall density of interneurons was determined by counting GFP positive cells within the boundaries of dLGN. These measurements were obtained from 2 to 3 sections corresponding to the middle of dLGN (n = 3 mice; P7, P9, and P26). We also examined whether interneurons showed a preference between binocular and monocular regions of dLGN. To accomplish this we made ipsilateral eye injections of cholera toxin subunit B (CTB) conju\n",
      "Values:  [ 7.  9. 26.]\n",
      "Unit:  day\n",
      " \n",
      "Number:  9\n",
      "Text:  The experiments were performed using brain slices prepared from 21-day-old rats.\n",
      "Context:  ites. Because of this, distal synaptic inputs could significantly depolarize the soma. Parts of this work have been published previously in abstract form (Perreault et al. 2000). Methods Preparation  The experiments were performed using brain slices prepared from 21-day-old rats. Some of the present material (half of the cells) has been used in a previous study (Perreault et al. 2003). All procedures were approved by the National Animal Research Authority in Norway. The anima\n",
      "Values:  21.0\n",
      "Unit:  day\n",
      " \n",
      "Number:  10\n",
      "Text:  Adult (6–12 months old) Wistar albino control rats (n = 5)\n",
      "Context:  Committee of Marmara University.  in TBST 7.6 and then with deionized water and subjected  \f",
      "Safiye Çavdar, et al.  Comparison of numbers of interneurons in three thalamic nuclei of normal and epileptic rats  453  Fig. 1. Coronal vibratome sections cut at 300 μm, showing thalamic areas sampled for the study of the lateral geniculate (LGN), ventral posteromedial (VPM) and ventral posterolateral (VPL) nuclei. The section chosen for each sample was neither t\n",
      "Values:    [6.0 - 12.0] \n",
      "Unit:  month\n",
      " \n",
      "Number:  11\n",
      "Text:  Data for IT, IA, and IK2 were derived from whole cell voltage- clamp studies obtained from acutely isolated ventroposterior relay neurons from young rats (7- 14 days old) (Coulter et al. 1989; Huguenard et al. 199 1; Huguenard and Prince 1992).\n",
      "Context:  s the time constant of deactivation of single m gates and therefore 7, was determined as 2 X 7,ail (where Vtair is in the membrane potential range where m, approaches zero; Hagiwara and Ohmori 1982). Data for IT, I*, and IK2 were derived from whole cell voltageclamp studies obtained from acutely isolated ventroposterior relay neurons from young rats (7- 14 days old) (Coulter et al. 1989; Huguenard et al. 199 1; Huguenard and Prince 1992). With the exception of studies characterizing the temperature dependence of the ionic currents, all isolated cell experiments were performed at room temperature (23-25°C) and were compensated for capaci\n",
      "Values:    [7.0 - 14.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  12\n",
      "Text:  from postnatal day 18–47\n",
      "Context:  rons are distinct from first order thalamic neurons because their response properties may be driven by cortical rather than subcortical inputs (Guillery 1995). METHODS  Long Evans rats ranging in age from postnatal day 18 – 47 were anesthetized with halothane and killed by decapitation. The brain was excised and a block of tissue containing the thalamus was removed, and placed in a cold (4°C) oxygenated solution of artif\n",
      "Values:    [18.0 - 47.0] \n",
      "Unit:  d\n",
      " \n",
      "Number:  13\n",
      "Text:  The activation of I NaP could be well fitted with a Boltzmann curve (Fig. 5B 3 ), charac- terized by a k of 9.09 and a V 1/2 (256.93 mV\n",
      "Context:  potential, because at potentials more than 210 mV an outward current with properties similar to those described by Alzheimer (1994) in pyramidal neurons of the sensorimotor cortex was also activated. The activation of INaP could be well fitted with a Boltzmann curve (Fig. 5B3), characterized by a k of 9.09 and a V1/2 (256.93 mV) that was more negative than that of INa (compare Fig. 1 A3, B2). To compare INaP  activation in different conditions (see below) we also fitted the data of each neuron with a Boltzmann curve. From this an\n",
      "Values:    [7.0 - 11.0] \n",
      "Unit:  days\n",
      " \n",
      "Number:  14\n",
      "Text:  Sprague–Dawley rats (Harlan, Indianapolis, IN; four to 10 weeks old; 100–300 g) were deeply anesthetized using halothane.\n",
      "Context:   slices, varying the standard LTS current-clamp protocol in order to take account of the altered voltage-dependent characteristics of IT in interneurons. 58 EXPERIMENTAL PROCEDURES  Slice preparation Sprague–Dawley rats (Harlan, Indianapolis, IN; four to 10 weeks old; 100–300 g) were deeply anesthetized using halothane. A minimum number of animals were used to obtain the necessary results and the animals were not allowed to suffer in any way. After decapitation, the brain was quickly removed into cold (6–8⬚C) physio\n",
      "Values:    [4.0 - 10.0] \n",
      "Unit:  week\n",
      " \n",
      "Number:  15\n",
      "Text:  Wistar rats (aged 28–29 d)\n",
      "Context:  n Animal Welfare Act and approved by the Institutional Animal Care and Use Committee of the Max Planck Florida Institute for Neuroscience. Histology was conducted as reported previously (24). Brieﬂy, Wistar rats (aged 28–29 d) were perfused transcardially, and brains were removed and ﬁxed with paraformaldehyde. For neuron counting in cortex, 43–48 consecutive Vibratome sections of 50 μm thickness were cut tangentially to v\n",
      "Values:    [28.0 - 29.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  16\n",
      "Text:  Rats of either sex (postnatal days 14-25)\n",
      "Context:  ctive functional interaction, such that the generation of regenerative low-threshold Ca2+ responses is promoted in relay cells and prevented under normal conditions in local circuit neurones. METHODS Rats of either sex (postnatal days 14-25) were deeply anaesthetized with halothane and decapitated. A block of tissue containing the LGN was removed and placed in 5°C physiological saline, containing (mM): NaCl, 126; KCl, 2-5; MgSO4, 2; NaH2\n",
      "Values:    [14.0 - 25.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  17\n",
      "Text:  Balb/c mice (30 – 40 days of age)\n",
      "Context:  l surgical procedures were approved by the Institutional Animal Care and Use Committee at the University of Illinois. All animals were housed in animal care facilities approved by the Association for Assessment and Accreditation of Laboratory Animal Care International. Every attempt was made to minimize the number of animals used and to reduce suffering at all stages of the study. Physiological recordings. Balb/c mice (30 – 40 da\n",
      "Values:    [30.0 - 40.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  18\n",
      "Text:  All in vitro recordings were obtained in TC neurons from the ventrobasal nucleus of young rats [postnatal day 8 (P8)–P15]\n",
      "Context:  c Relay Cells  J. Neurosci., May 15, 1998, 18(10):3574–3588 3575  MATERIALS AND METHODS In vitro recordings. The present study focuses on neurons from the ventrobasal nucleus of the thalamus of rats. All in vitro recordings were obtained in TC neurons from the ventrobasal nucleus of young rats [postnatal day 8 (P8)–P15], using either intact slice preparation or acutely dissociated TC cells. All current-clamp recordings were at a temperature of 34 –36°C, whereas voltage-clamp recordings were done at 24°C. The methods\n",
      "Values:    [8.0 - 15.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  19\n",
      "Text:  at the age of 24 days\n",
      "Context:  Rad, Hercules, CA; 1:4000).  RESULTS j/j  CTSD  Brains Show Widespread Atrophy  Macroscopically, the brains of CTSDj/j mice seemed relatively normal but proportionately smaller than those of controls at the age of 24 days. Consistent with this observation, Nissl-stained sections revealed widespread atrophy of the CNS in the affected mice (Fig. 1A). Cavalieri estimates of regional volume showed significant effects upon\n",
      "Values:  24.0\n",
      "Unit:  days\n",
      " \n",
      "Number:  20\n",
      "Text:  postnatal days 10-20\n",
      "Context:  Ca2+ in TC cells using a combination of whole-cell patch-clamp and imaging techniques in the slice preparation of the ventrobasal thalamic complex (VB) in vitro.  METHODS Preparation Long-Evans rats (postnatal days 10-20) were deeply anaesthetized with halothane and decapitated. A block of tissue containing the VB was removed and placed in ice cold saline, containing (mM): sucrose, 200; Pipes, 20; KCl, 2-5; NaH2PO4, \n",
      "Values:    [10.0 - 20.0] \n",
      "Unit:  days\n",
      " \n",
      "Number:  21\n",
      "Text:  All rats were male and 4 weeks old at the start of the study.\n",
      "Context:  ess if MAinduced structural and functional changes in the brain were associated with neuroinflammation.  Methods and Materials Animals Sprague Dawley (SD) rats were purchased from Taconic farms (NY). All rats were male and 4 weeks old at the start of the study. Animals were individually housed in clear plexi-glass cages,  PLOS ONE | DOI:10.1371/journal.pone.0155457 June 8, 2016  2 / 18  \f",
      "Structural and Functional Effects of Chronic MA  with wire covers unde\n",
      "Values:  4.0\n",
      "Unit:  week\n",
      " \n",
      "Number:  22\n",
      "age) were used for this study.les: 7.7 and 9.3 years of 5.3\n",
      "Context:  xed at 4\u0001 C for 4 weeks prior to Nissl staining with thionin. All other series were collected in tissue collection solution and kept at \u000270\u0001 C until further processing (Lavenex et al., 2009a).  Rats  Four rhesus monkeys, Macaca mulatta, (two males: 5.3 and 9.4 years of age; two females: 7.7 and 9.3 years of age) were used for this study. Monkeys were born from multiparous mothers and raised at the California National Primate Research Center (CNPRC). They were maternally reared in 2,000 m2 outdoor enclosures and lived in large social \n",
      "Values:  [9.4 9.3 7.7 5.3]\n",
      "Unit:  years\n",
      " \n",
      "Number:  23\n",
      "Text:  between 3 and 5 months of age\n",
      "Context:  synaptic organization of striatal MSNs is altered in cbln1 null mutant mice.  2526  Manufacturer  MATERIALS AND METHODS Animals  The cbln1\u0001/\u0001 mice and their wildtype littermates (Hirai et al., 2005), between 3 and 5 months of age, were group housed with food and water freely available. The cbln1\u0001/\u0001 mice were backcrossed to the C57Bl/6J strain for more than nine generations. Sprague–Dawley rats (Harlan Sprague-Dawley; Indianap\n",
      "Values:    [3.0 - 5.0] \n",
      "Unit:  months\n",
      " \n",
      "Number:  24\n",
      "Text:  14- to 21-day-old rats\n",
      "Context:  vement of calmodulin in CDI in relay neurons.  studies determining the expression of calbindin-D28K (CB), calretinin (CR) and parvalbumin (PV) were performed on dLGN slices (60 lm thick sections from 14to 21-day-old rats). Alternate sections were Nissl-stained and revealed an overall density of neurons in the dLGN of 5,434±326 cells/mm2 (measured in six slices from three animals). Western blot analysis of dLGN tissue u\n",
      "Values:    [14.0 - 21.0] \n",
      "Unit:  d\n",
      " \n",
      "Number:  25\n",
      "Text:  About 6 male C57/BL6 mice (60–65 days of age) were used.\n",
      "Context:  e carried out in the Autonoma University of Madrid, in accordance with the European Community Council Directive 2010/63/UE and approved by the University’s Bioethics Committee.  Surgery and Perfusion About 6 male C57/BL6 mice (60–65 days of age) were used. Animals were housed in pairs in 20 by 30 cm cages containing some toys, and provided chow and water ad libitum under a 12 h light/dark cycle. Anesthesia was induced with ketamine (0.075 mg/g, i.p.) a\n",
      "Values:    [60.0 - 65.0] \n",
      "Unit:  day\n",
      " \n",
      "Number:  26\n",
      "Text:  postnatal days 12-–29\n",
      "Context:  1,2-dimethyl-6-(methylamino) pyridinium chloride; Biotrend, Cologne, Germany] was directly dissolved in the external recording solution.  Preparation  Computer simulations with NEURON  Rats and mice (postnatal days 12-–29) were anesthetized with halothane, decapitated, and used for electrophysiological, immunohistochemical, and molecular biological analysis. A block of tissue containing the thalamus was removed and pl\n",
      "Values:    [12.0 - 29.0] \n",
      "Unit:  d\n",
      " \n",
      "Number:  27\n",
      "Text:  ∼P265\n",
      "Context:  measures from a rotarod task, Morris water maze, a social preference task, and male vocalization behaviors in a socially relevant context [56]. 2.2. Histology Following the end of behavioral testing (∼P265), subjects were weighed, anesthetized using a cocktail of ketamine (100 mg/kg) and xylazine (15 mg/kg) and transcardially perfused using a 0.9% saline solution followed by 4% paraformaldehyde. Brains\n",
      "Values:  265.0\n",
      "Unit:  days\n",
      " \n",
      "PARAMETER NAME: resting_membrane_potential\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Number:  1\n",
      "Text:  resting membrane potential: 262 6 2 mV apparent steady-state input resistance: 191 6 24 MV; n 5 10)\n",
      "Context:  ing current-clamp recordings from adult rat dLGN slices (Fig. 8). The membrane properties of these neurons were similar to those described previously for TC neurons in identical recording conditions (resting membrane potential: 262 6 2 mV;  \f",
      "Parri and Crunelli • INa and INaP in Thalamic Neurons  apparent steady-state input resistance: 191 6 24 MV; n 5 10). The effect on tonic firing was investigated by recording from TC neurons held at membrane potentials more than or equal to 260 mV, from wher\n",
      "Values:  -62.0 +/- 2.0  \n",
      "Unit:  mV\n",
      " \n",
      "Number:  2\n",
      "Text:  The resting potential of interneurones (-52 + 1 mV, n = 24) was found to be significantly (P < 0 05, T= 9 09) more positive than that of TC neurones (-63 + 1 mV, n = 30).\n",
      "Context:  onstant of interneurones (36-8 + 3-8 ms, n = 24), calculated by the fitting of a mono-exponential function to small (< 10 mV)  current relationships, whilst those of TC  neurones  Membrane properties The resting potential of interneurones (-52 + 1 mV, n = 24) was found to be significantly (P < 0 05, T = 9 09) more positive than that of TC neurones (-63 + 1 mV, n = 30). Of our sample, 13% of interneurones were found to fire action potentials spontaneously, when no current was injected through the recording electrode. The input resistance of interneurones (164 + 15 \n",
      "Values:  -52.0 +/- 1.0  (n=24.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  3\n",
      "Text:  n vitro studies (209, 171) demonstrated that TC cells possess two different firing modes. In the “tonic” mode, near the resting membrane potential (approximately 􏰁60 mV)\u0000\n",
      "Context:  ound burst” or LTS. Andersen and Eccles (9) were the first to show that TC cells display bursts of action potentials tightly correlated with the offset of inhibitory postsynaptic potentials (IPSPs). In vitro studies (209, 171) demonstrated that TC cells possess two different firing modes. In the “tonic” mode, near the resting membrane potential (approximately Ϫ60 mV), the relay neuron fires trains of action potentials at a frequency proportional to the amplitude of the injected current (Fig. 2A, left panel). This is similar to the response of many other neurons an\n",
      "Values:  -60.0\n",
      "Unit:  mV\n",
      " \n",
      "Number:  4\n",
      "Text:  A representa- tive sample of 10 of these neurons exhibited an average resting membrane potential of -61+/- 6 (SD) mV and apparent input resistance of 137 +/- 25 MOhm\n",
      "Context:   mV, indicating that activation of potassium currents mediates the AHP generation.  RESULTS  Intracellular recordings were obtained from 147 PGN cells in ferret geniculate slices maintained in vitro. A representative sample of 10 of these neurons exhibited an average resting membrane potential of 061 { 6 (SD) mV and apparent input resistance of 137 { 25 MV, as measured by the electrotonic response to a 0.1- to 0.2-nA hyperpolarizing current pulse at resting membrane potentials. During the generation of spindle waves, PGN cells received cyclic barrages of exci\n",
      "Values:  -61.0 +/- 6.0  (n=10.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  5\n",
      "Text:  Measurements of these responses revealed that input resistance (55 \u0003 19M\u0002; n \u0004 94), resting membrane potential (\u000560 \u0003 4 mV; n \u0004 67), and spike amplitude (64 \u0003 7mV; n \u0004 80) \n",
      "Context:  rane properties of LPN cells The voltage responses to square-wave current pulses were used to explore the passive and active membrane properties of LPN relay neurons. Examples are shown in Figs. 2–5. Measurements of these responses revealed that input resistance (55 Ϯ 19M⍀; n ϭ 94), resting membrane potential (Ϫ60 Ϯ 4 mV; n ϭ 67), and spike amplitude (64 Ϯ 7mV; n ϭ 80) are similar to those of LGN relay neurons (Williams et al. 1996). An examination of I-V relations also indicated substantial nonlinearities in the voltage responses to current pulses (Fig. 2E). Indeed\n",
      "Values:  -60.0 +/- 4.0  (n=67.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  6\n",
      "Text:  There was no significant difference in the Vrest between neurons in the ventral and dorsal MGB (dorsal: -59.06 ± 0.56 mV, n = 36; ventral: -58.41 ± 0.74 mV, n = 22, P[0.05)\n",
      "Context:   were from the ventral and dorsal parts of the MGB (elliptical region in Fig. 1A). None of the neurons with a stable resting membrane potential (Vrest; more negative than -52 mV) fired spontaneously. There was no significant difference in the Vrest between neurons in the ventral and dorsal MGB (dorsal: -59.06 ± 0.56 mV, n = 36; ventral: -58.41 ± 0.74 mV, n = 22, P [ 0.05), which is consistent with the findings of Bartlett and Smith [5]. Action potentials were evoked by a robust RD (Fig. 2A). In most cases, an MGB neuron exhibited various patterns of rebound discharg\n",
      "Values:  -58.41 +/- 0.74  (n=22.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  7\n",
      "Text:  The resting potential of the relay cells 71.4 +/- 3.1 mV, and that of the interneurons was 69.1+/-  3.6 mV.\n",
      "Context:  brane timeconstant (101.2&19.3 vs 22.5&7.1 MD). These characteristics alone were sufficient to distinguish unambiguously the two cell types prior to subsequent morphological confirmation (see below). The resting potential of the relay cells \"71.4&3.1 mV, and that of the interneurons was \"69.1&3.6 mV. The morphology of nine relay cells and all 11 interneurons was recovered in subsequent histological processing, and in each case, the physiologicallydetermined classification was confirmed (cf. Refs 11, 14\n",
      "Values:  -69.1 +/- 3.6  (n=11.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  8\n",
      "Text:  There was no significant difference in the Vrest between neurons in the ventral and dorsal MGB (dorsal: -59.06 ± 0.56 mV, n = 36;\n",
      "Context:   were from the ventral and dorsal parts of the MGB (elliptical region in Fig. 1A). None of the neurons with a stable resting membrane potential (Vrest; more negative than -52 mV) fired spontaneously. There was no significant difference in the Vrest between neurons in the ventral and dorsal MGB (dorsal: -59.06 ± 0.56 mV, n = 36; ventral: -58.41 ± 0.74 mV, n = 22, P [ 0.05), which is consistent with the findings of Bartlett and Smith [5]. Action potentials were evoked by a robust RD (Fig. 2A). In most cases, an MGB neuron exh\n",
      "Values:  -59.06 +/- 0.56  (n=36.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  9\n",
      "Text:  We found no differences between CaV3.1+/+ (n = 12) and CaV3.1−/− (n = 14) TC neurons in membrane capacitance (141.2 ± 15.6 pF vs. 137.4 ±10.5 pF), resting membrane potential (−59.11 ± 5.34 mV vs. −57.54 ± 6.31 mV), or membrane resistance (219.5 ± 48.2 MΩ vs. 175.4 ± 38.2 MΩ).\n",
      "Context:  TC neurons rather than to a loss of lowthreshold burst ﬁring by deletion of CaV3.1 T-type channels. To do this, we used the whole-cell patch-clamp technique to measure the excitability of TC neurons. We found no differences between CaV3.1+/+ (n = 12) and CaV3.1−/− (n = 14) TC neurons in membrane capacitance (141.2 ± 15.6 pF vs. 137.4 ±10.5 pF), resting membrane potential (−59.11 ± 5.34 mV vs. −57.54 ± 6.31 mV), or membrane resistance (219.5 ± 48.2 MΩ vs. 175.4 ± 38.2 MΩ). We then measured hyperpolarization-activated cyclic nucleotide-gated channel-mediated current (Ih) in TC neurons at the tail current at 0.7 s after a test voltage pulse (Fig. 5A) to minimize the capa\n",
      "Values:  -59.11 +/- 5.34  \n",
      "Unit:  mV\n",
      " \n",
      "Number:  10\n",
      "Text:  The resting potential of the relay cells 71.4 +/- 3.1 mV, and that of the interneurons was 69.1+/-  3.6 mV.\n",
      "Context:  brane timeconstant (101.2&19.3 vs 22.5&7.1 MD). These characteristics alone were sufficient to distinguish unambiguously the two cell types prior to subsequent morphological confirmation (see below). The resting potential of the relay cells \"71.4&3.1 mV, and that of the interneurons was \"69.1&3.6 mV. The morphology of nine relay cells and all 11 interneurons was recovered in subsequent histological processing, and in each case, the physiologicallydetermined classification was confirmed (cf. Refs 11, 14\n",
      "Values:  -71.4 +/- 3.1  (n=37.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  11\n",
      "Text:  Resting membrane potential (RMP) in the interneurons was -67.7 +/- 5.1 mV\n",
      "Context:  both about five times higher than those seen in geniculate TC cells (118.7 ^ 45.7 MV and 22.5 ^ 7.1 ms; see Zhu and Uhlrich 90) and four to five times higher than reported previously in interneurons. Resting membrane potential (RMP) in the interneurons was ⫺67.7 ^  1447  5.1 mV, compared to ⫺ 71.4 ^ 3.1 mV for TC cells. 90 Figure 1 also illustrates a key difference between the two cell types. TC cells always showed burst firing on release from hyperpolarization. 39,40 \n",
      "Values:  -67.7 +/- 5.1  (n=102.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  12\n",
      "Text:  Stable intracellular recordings from cells in paralaminar nuclei could last for several hours, and resting membrane potentials (–65 \u0005 6.7 mV)\n",
      "Context:   parent cell (Fig. 5) or could come off the main axon at more distant sites to innervate other paralaminar regions (Fig. 4). None of the collaterals was seen entering MGV or MGD.  Physiology General. Stable intracellular recordings from cells in paralaminar nuclei could last for several hours, and resting membrane potentials (– 65 Ϯ 6.7 mV) were not significantly different from those of cells in MGV and MGD that we previously reported (– 62.2 Ϯ 8.8 mV). Action potential amplitudes of paralaminar cells (72.1 Ϯ 10.9 mV) were  \f",
      "Fig. 4. El\n",
      "Values:  -65.0 +/- 6.7  \n",
      "Unit:  mV\n",
      " \n",
      "Number:  13\n",
      "Text:  Stable intracellular recordings from cells in paralaminar nuclei could last for several hours, and resting membrane potentials (–65 \u0005 6.7 mV) were not significantly different from those of cells in MGV and MGD that we previously reported (–62.2 \u0005 8.8 mV).\n",
      "Context:   parent cell (Fig. 5) or could come off the main axon at more distant sites to innervate other paralaminar regions (Fig. 4). None of the collaterals was seen entering MGV or MGD.  Physiology General. Stable intracellular recordings from cells in paralaminar nuclei could last for several hours, and resting membrane potentials (– 65 Ϯ 6.7 mV) were not significantly different from those of cells in MGV and MGD that we previously reported (– 62.2 Ϯ 8.8 mV). Action potential amplitudes of paralaminar cells (72.1 Ϯ 10.9 mV) were  \f",
      "Fig. 4. Elongate cell in a paralaminar nucleus. A: Camera lucida drawing of the cell body, dendritic tree, axon (a), and axo\n",
      "Values:  -62.2 +/- 8.8  \n",
      "Unit:  mV\n",
      " \n",
      "Number:  14\n",
      "Text:  The resting potential of interneurones (-52 + 1 mV, n = 24) was found to be significantly (P < 0 05, T= 9 09) more positive than that of TC neurones (-63 + 1 mV, n = 30).\n",
      "Context:  onstant of interneurones (36-8 + 3-8 ms, n = 24), calculated by the fitting of a mono-exponential function to small (< 10 mV)  current relationships, whilst those of TC  neurones  Membrane properties The resting potential of interneurones (-52 + 1 mV, n = 24) was found to be significantly (P < 0 05, T = 9 09) more positive than that of TC neurones (-63 + 1 mV, n = 30). Of our sample, 13% of interneurones were found to fire action potentials spontaneously, when no current was injected through the recording electrode. The input resistance of interneurones (164 + 15 \n",
      "Values:  -63.0 +/- 1.0  (n=30.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  15\n",
      "Text:  For these interneurons the input resistance was 605 +/- 209 MOhm, the time constant was 93.1 +/- 24.2 ms and the resting membrane potential was -68.6 +/- 4.8 mV.\n",
      "Context:  study, the same criteria (i.e. input resistance larger than 300 MV and/or time constant longer than 50 ms) were used to identify interneurons prior to subsequent morphological confirmation (Fig. 1B). For these interneurons the input resistance was 605 ^ 209 MV, the time constant was 93.1 ^ 24.2 ms and the resting membrane potential was 268.6 ^ 4.8 mV. The morphology of all 54 interneurons was recovered, which confirmed that the recordings were obtained from interneurons. 15–17,37,38,57,58 A representative morphological example is illustrated in Fig. 1A. I\n",
      "Values:  -68.6 +/- 4.8  (n=54.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  16\n",
      "Text:  The resting membrane potential for thirty-seven TC neurones was -61P9 + 0 7 mV,\n",
      "Context:  e network (Fig. 6B), with each fibre exhibiting varicosities along their entire length  (Fig. 6C). The membrane properties of TC neurones Resting membrane potential and delta oscillation. A mean stable resting membrane potential of -61-9 + 0 7 mV was measured for thirty-seven of the forty-one TC neurones studied (Table 2). The four remaining TC neurones had nonstable membrane potentials in the absence of injected DC, owing to the-presence of delta (or pacemaker) oscillation \n",
      "Values:  -61.9 +/- 0.7  (n=37.0)\n",
      "Unit:  mV\n",
      " \n",
      "Number:  17\n",
      "Text:  resting membrane potential was about −70.5 mV\n",
      "Context:  rovided by Destexhe and Huguenard was of a fairly large cell (www.cnl.salk.edu/∼alain/). The somatic input time constant, τ in , measured during relaxation to resting voltage following a small depolarizing step, was approximately 19 ms, and resting membrane potential was about −70.5 mV. Relay cells are distinguished by a particularly large complement of currents active at rest, including the low threshold (T-type) calcium channel (Jahnsen & \n",
      "Values:  -70.5\n",
      "Unit:  mV\n",
      " \n",
      "Number:  18\n",
      "Text:  resting membrane potential of −70 mV\n",
      "Context:  /−, n = 6). (D) PCR analysis of tail DNA to identify WT, heterozygous, and homozygous animals. PCR primers used are identiﬁed in SI Materials and Methods.  were generated upon depolarization from the resting membrane potential of −70 mV (16). In contrast, in nRt cells from CaV3.3−/− mice, repetitive burst discharges were absent, whereas tonic ﬁring was unaltered (Fig. 2A and Table S1). Passive membrane properties did not differ betw\n",
      "Values:  -70.0 +/- 4.0  \n",
      "Unit:  mV\n",
      " \n"
     ]
    }
   ],
   "source": [
    "from nat.annotationSearch import ParameterSearch, ConditionAtom\n",
    "\n",
    "parameters = flatten_list([annot.parameters for annot in annotations])        \n",
    "paramTypes = pd.Series([param.typeId for param in parameters])\n",
    "paramNames = [getParameterTypeNameFromID(typeId) for typeId in paramTypes.value_counts().iloc[:5].index]\n",
    "\n",
    "for paramName in paramNames:\n",
    "    print(\"PARAMETER NAME:\", paramName)\n",
    "    searcher = ParameterSearch(repo_dir)\n",
    "    searcher.setSearchConditions(ConditionAtom(\"Parameter name\", paramName))\n",
    "    searcher.expandRequiredTags = True\n",
    "    searcher.onlyCentralTendancy = False\n",
    "    searcher.contextLength       = 200\n",
    "    resultDF = searcher.search()\n",
    "    no = 0\n",
    "    for _, row in resultDF.iterrows():\n",
    "        if row[\"obj_annotation\"].type == \"text\":\n",
    "            no += 1\n",
    "            print(\"Number: \", no)\n",
    "            print(\"Text: \", row[\"Text\"].replace(\"\\n\", \" \"))\n",
    "            print(\"Context: \", row[\"Context\"].replace(\"\\n\", \" \"))\n",
    "            print(\"Values: \", row[\"Values\"])\n",
    "            print(\"Unit: \", row[\"Unit\"])\n",
    "            print(\" \")"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  }
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